Synaptotagmin Antikörper (pSer309)

Produktnummer ABIN361497

Dieser Anti-Synaptotagmin Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von Synaptotagmin in WB, ELISA und ICC. Geeignet für Ratte. Dieses Primary Antibody wurde in 5+ Publikationen zitiert.

Kurzübersicht für Synaptotagmin Antikörper (pSer309) (ABIN361497)

Target: Synaptotagmin (SYT)

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Synaptotagmin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Produktdetails anti-Synaptotagmin Antikörper

Bindungsspezifität: pSer309

Spezifität: Specific for endogenous levels of the ~62 kDa synaptotagmin protein phosphorylated at Ser 309. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Kreuzreaktivität: Ratte

Homologie: bovine, canine, chicken, human, mouse, non-human primate, zebra fish

Produktmerkmale: Our Synaptotagmin (Ser309) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects Synaptotagmin (Ser309) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, ICC.

Aufreinigung: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser309 of rat synaptotagmin, conjugated to keyhole limpet hemocyanin (KLH).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Dilution Range: ICC: 1:400

Dilution Range: WB Brain: 1:1000

Dilution Range: WB: 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Lagerung: -20 °C

Informationen zur Lagerung: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Haltbarkeit: 12 months

Referenzen für anti-Synaptotagmin Antikörper (ABIN361497)

Leal-Ortiz, Waites, Terry-Lorenzo, Zamorano, Gundelfinger, Garner: "Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis." in: The Journal of cell biology, Vol. 181, Issue 5, pp. 831-46, (2008) (PubMed).

Poskanzer, Marek, Sweeney, Davis: "Synaptotagmin I is necessary for compensatory synaptic vesicle endocytosis in vivo." in: Nature, Vol. 426, Issue 6966, pp. 559-63, (2003) (PubMed).

Wang, Lu, Bai, Chang, Martin, Chapman, Jackson: "Different domains of synaptotagmin control the choice between kiss-and-run and full fusion." in: Nature, Vol. 424, Issue 6951, pp. 943-7, (2003) (PubMed).

Fernández-Chacón, Königstorfer, Gerber, García, Matos, Stevens, Brose, Rizo, Rosenmund, Südhof: "Synaptotagmin I functions as a calcium regulator of release probability." in: Nature, Vol. 410, Issue 6824, pp. 41-9, (2001) (PubMed).

Hilfiker, Pieribone, Nordstedt, Greengard, Czernik: "Regulation of synaptotagmin I phosphorylation by multiple protein kinases." in: Journal of neurochemistry, Vol. 73, Issue 3, pp. 921-32, (1999) (PubMed).

Details zu Synaptotagmin

Target: Synaptotagmin (SYT)

Andere Bezeichnung: Synaptotagmin

Hintergrund:

Synonyms: DKFZp781D2042 antibody, FLJ42519 antibody, P65 antibody, SVP65 antibody, synaptotagmin 1 antibody, Synaptotagmin I antibody, SYT antibody, SYT1 antibody, SytI antibody

Description Logic: Our Synaptotagmin (Ser309) rabbit polyclonal phosphospecific primary antibody is produced . It detects Synaptotagmin (Ser309) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, ICC.

Gene Name Alternatives: SYT1

Molekulargewicht: 60 kDa/62 kDa

Gen-ID: 25716

UniProt: P21707